Rapid purity check and screening superdex 75 5150 gl, superdex 200 increase 5150 gl and superose 6 increase 5150 gl are short columns with small bed volumes that are suitable for rapid protein homogeneity analyses or purity checks. When time is limited and small buffer and sample consumption is important, this is the column of choice. For more than forty years since the introduction of sephadex, gel filtration has played a key role in the purification of enzymes, polysaccharides, nucleic acids, proteins and other. Superdex 200 prep grade pg are prepacked xk columns designed for preparative gel filtration. Bpg 200 superdex 30 prep grade bpg 200 superdex 200 prep grade bpg 200 superdex 75 prep grade fig. Superdex 200 10300 gl chromatographic separation column from. All books are in clear copy here, and all files are secure so dont worry about it. Hiload superdex columns, 200 pg, ge healthcare chromatography columns superdex prep grade pg is produced by covalent bonding of dextran to highly crosslinked agarose. The department of physiology and biophysics school of. We dont know when or if this item will be back in stock.
Intended use superdex 200 increase 5150 gl and superdex 200 increase 10300 gl columns are intended for research use only, and shall not be. Superdex 75 prep grade and superdex 200 prep grade are ideal for all stages. Superdex 200 increase 10300 gl ge healthcare life sciences. Superdex 75 5150 gl, superdex 200 increase 5150 gl and. Instructions 56119099 ae gel filtration media superdex 30. Hiload 26600 superdex 200 pg ge healthcare, 28989336, column l. Highsample load 500 l separation of a mixture containing seven. Cookies this website uses cookies, which are necessary to is functionling and required to achieve the purposes illustrated in the cookie policy.
Ge, the ge monogram, akta, superdex, and tricorn are trademarks of general electric company. Ge healthcare sepharose high performance capto impres superdex 200 pg capto q, s, deae sephacryl s200 hr sepharose 4 fast flow sepharose 6 fast flow sepharose big beads mabselect, mabselect sure 160 120 80 40 0 0 20 40 60 80 100 120 140 160 180 200 apparent pore diameter according to isec nm typical particle size m mabselect xtra. Methods, which can be ordered from ge healthcare, or the method handbook. Akta basic 10 chromatography system amersham biosciences ab, uppsala, sweden was used to run all chromatography experiments. Superdex prep grade is a composite matrix of dextran and crosslinked agarose. Purification of globular actin from rabbit muscle and pyrene fluorescent assays to investigate actin dynamics in vitro. Instruction 29027271 ah size exclusion chromatography columns 28990945 superdex 200 increase 5150 gl 28990944 superdex 200 increase 10300 gl read these instructions carefully before using the columns. I may be wrong on this, i use superdex 200 for the most part. Superdex 75 10300 gl prepacked column for smallscale preparative purification g to mg or characterization of proteins in the molecular range 3000 to 70 000, like recombinant, tagged proteins.
Superdex 200 increase 5150 gl prepacked column for rapid purity check and screening by size exclusion chromatography for proteins with molecular weights between 10 000 and 600 000, such as antibodies. Columns up to 1600 mm in diameter packed with protein a. Cleaning gel filtration column which is the best cleaning. Superdex prep grade pg is produced by covalent bonding of dextran to highly crosslinked agarose. The superdex 200 10300 gl column from ge healthcare is a high performance gel filtration column. Elution volumes for globular proteins of various molecular weights on superdex 75 increase 10300 blue, superdex 200 increase 10300 green, and superose 6 increase 10300 orange. Size exclusion chromatography columns and media ge. Direct selection of a human antibody fragment directed against the tumor tcell epitope hlaa1magea1 from a nonimmunized phagefab library. Hiload superdex 200 pg preparative sec columns ge healthcare. Superdex prep grade is a composite matr ix of dextran and highly crosslinked agarose. As shown in figures 4 a and b, the detection signal from the run with superdex 200 increase 3. Protein purification chromatography calibration of. Maintenance and cleaning of size exclusion chromatography columns.
Ge healthcare hiload 16600 superdex 200 pg hiload 1660. Superdex 200 increase smallscale sec columns cytiva. Superdex prep grade is a matrix of dextran and highly crosslinked agarose. A range of prepacked columns is available to suit a variety of preparative and analytical applications table 11. Superdex 200 increase columns superdex 200 increase prepacked columns fig 1 are designed for size exclusion chromatography sechigh resolution gel filtration in smallscale g to mg, preparative purification, as well as for characterization and analysis of proteins with molecular weights m r from 10 000 to 600 000. You need to choose a quantity before adding items to cart. Read these instructions carefully before using the columns. Ge healthcare life sciences hiload 16600 and 26600 superdex 30 prep grade. Instruction 29027271 ah size exclusion chromatography columns. Designed for highresolution protein fractionation at preparative scales. Filled with stationary phase compositions or resins, the prepacked options reduce the preparing time required. Ion exchange chromatography ac affinity chromatography ge format guide type of purification manual purification manual or system purification system purification format h pp g s 96l spin columns. Ideal for the polishing step in a purification procedure. Ge states in the s200 manual, that the sephadex flavour would require much longer equilibration times advertising, that s200 would be much speeding up the process.
Superdex 200 increase 10300 gl ge healthcare, 28990944. Ge healthcare hiload 16600 superdex 200 pg is ideal for highresolution preparative gel filtration chromatography. Superdex gel filtration media are listed in appendix c, table 1. Ge life sciences 28989335 hiload 16600 superdex 200. The separation properties of these media are determined by the dextran componentchromatography columns separate molecules within mixtures during gas or liquid methods. Superdex 200 increase 5150 gl ge healthcare, 28990945, column l. Hiload 16600 and 26600 superdex 30 prep grade hiload 16. With their high physical and chemical stability, very high batchtobatch reproducibility, and regulatory support file backup, superdex 30 prep grade, superdex 75 prep grade and superdex 200 prep grade are ideal for all stages of an industrial scale operation from research and.
Hiload superdex 200 pg columns m r 10 000600 000 hiprep sephacryl s200 hr columns m r 5 000250 000 hiprep sephacryl s100 hr columns m r 1 000100 000 hiload superdex 75 pg columns m r 3 00070 000 hiload superdex 30 pg columns m r 10300 gl prepacked tricorn columns for size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Ge healthcare superdex 200 increase 10300 gl prepacked tricorn columns for size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Ge healthcare hiload 26600 superdex 200 pg hiload 2660. These are prepacked glass columns intended for use in gel filtration of proteins, peptides and other biomolecules. For the range of protein sizes youre talking about, i believe a superdex 75 column might serve your needs better than superdex 200. The chromatography medium in superdex 200 increase columns is based on a highflow agarose base. The steep selectivity of dextran and the high chemical and physical. Jan 12, 2010 the superdex 200 10300 gl column from ge healthcare is a high performance gel filtration column.
Prepacked with superdex 200 pg resin, these size exclusion chromatography columns. Hiload 26600 superdex 200 pg ge healthcare, 28989336. Hiload 16600 and 26600 superdex 30 prep grade hiload. Hiload 16600 and 26600 superdex 75 prep grade hiload 16600 and 26600 superdex 200 prep grade introduction hiload 16600 and 26600 superdex 30 prep grade, hiload 16600 and 26600 superdex 75 prep grade, and hiload 16600 and 26600 superdex 200 prep grade pg are prepacked xk columns designed for preparative size exclusion chromatography. Technical overview ge d200 technical overview 108 pages. Ethanol and especially sodium acetate affects the sedimentation prop erties of the resins and so it must be washed off completely before packing the column.
Ge healthcare life sciences data file 18110052 ae gel filtration hiload superdex 30 prep grade hiload superdex 75 prep grade hiload superdex 200 prep grade hiload columns are xk laboratory columns prepacked with superdex prep grade for gel filtration. Ge healthcare lifescience superdex 200 increase 10300 gl prepacked tricorn columns are designed for small scale preparative purification. Hiload superdex 30, 75, and 200 prep grade columns bring. Ge life sciences 28989335 hiload 16600 superdex 200 prep grade, prepacked column, 120ml currently unavailable. Superdex 200 prep grade pg are prepacked xk columns designed for preparative size exclusion chromatography. Reverse the flow direction and pump 69 ml for superdex 75 5150 gl and superdex 200 5150 gl 80100 ml for superdex 75 10300 gl, superdex 200 10300 gl, superdex peptide 10300 gl of well degassed eluent buffer through the column at a flow rate of 0. It has a separation range for molecules with mw between 10 000 and 600 000 da.
Superdex 75 10300 gl from ge healthcare selectscience. November 1966 general electric information systems division. Superdex pg is supplied in a storage solution of 0. Ge healthcare superdex 200 increase 10300 gl prepacked. We now have 2 superdex 200 gl columns in the lab, which is having a hard time. Superdex 200 increase 5150 gl ge healthcare, 28990945. Separations in two ranges from mr 3000 up to 600 000 globular proteins. Superdex 30 prep grade hiload 1660 and 2660 superdex 75 prep grade hiload 1660 and 2660 superdex 200 prep grade instructions 71502020 af high performance columns ge healthcare hiload introduction hiload 1660 and 2660 superdex 30 prep grade, superdex 75 prep grade, and superdex 200 prep grade are prepacked xk columns designed. Ive only used 75 once or twice, but i believe all the proteins youre talking about fall.
Superdex 200 increase is a nextgeneration, dextranagarose composite matrix for sec. Ge and ge monogram are trademarks of general electric company. The ge superdex 200 10300 gl is a prepacked gel filtration system. Ge healthcare size exclusion chromatography superdex 75. Superdex 200 10300 gl chromatographic separation column. Superdex prepacked columns are designed for highperformance, laboratoryscale separations of proteins, peptides, and other biomolecules according to size. The separation properties of these media are determined by the dextran component. Hiload superdex columns, 200 pg, ge healthcare vwr. Superdex 200 increase columns the wolfson centre for applied. Superdex 200 increase 10300 gl ge healthcare, 28990944, column l. Use for separating mabs from dimers and contaminants. With smaller and more rigid beads than their predecessors, superdex increase columns deliver higher resolution purification in shorter run times. Preface this manual has been prepared as a guide to operating the central processor for the ge225. Superdex 200 increase 5150 gl is for rapid size analysis of proteins.
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